Covina Discovery Lab Capabilities
- Production of high titer viral particles for transduction: Lentivirus (2nd/3rd Generation, VSV-G Pseudotyped); γ-retrovirus (VSV-G Pseudotyped)
- Quantification and validation of viral titers: p24 ELISA; Target cell transduction and flow cytometric analysis; RT qPCR
- Cell line generation: transcriptional reporters; target cell lines for cytotoxicity assays; cloning; stable cell line development
- Process development: primary T cell culture and engineering; PBMC and T cell isolation; T cell transduction; in vitro T cell expansion
- Proof of Concept demonstration studies: Phenotype and CAR-expression; T cell activation; T cell cytotoxicity; cytokine release
Covina Facility Fast Facts
6,000 sq. ft.
laboratory space
2,000 sq. ft.
overflow warehouse space for non-GMP material
Pilot scale clean room
for pre-clinical GLP batch development
Lab Equipment
- 3x BSCs(Class II A2)
- 6 x CO2 Incubators
- 30x 6’ Lab Benches
- LN2 storage and cell bank
- LCMS
- Spectrophotometer
- Miltenyi CliniMACS Prodigy®
- HPLC
- Live-Cell Analysis
- Sepax C-Pro
- FACS (Attune and Cytoflex)
- qPCR, ddPCR
- Barkey plasmatherm
- Luminex flow cytometer
- Incucyte® Live-Cell Analysis
Interested in Clinical Production? See Our cGMP Facility